Journal: Frontiers in Cellular Neuroscience

Article Title: Molecular and Functional Characterization of Neurogenin-2 Induced Human Sensory Neurons

doi: 10.3389/fncel.2020.600895

Figure Lengend Snippet: Primary and secondary antibody details and dilutions.

Article Snippet: Guinea pig anti-ASIC3 , AGP-052, Alomone , 1:500.

Techniques: Diagnostic Assay

Journal: Scientific Reports

Article Title: Vacuolar-ATPase-mediated muscle acidification caused muscular mechanical nociceptive hypersensitivity after chronic stress in rats, which involved extracellular matrix proteoglycan and ASIC3

doi: 10.1038/s41598-023-39633-1

Figure Lengend Snippet: Changes in RCS-induced decrease of MMWT by antagonists to ASIC3 and TRPs ( A ), in mRNAs expression of ASIC3 and TRPV1 ( B ) and in protein of ASIC3 in DRGs after RCS ( C , D ). ( A ) The presentation methods are the same as those in Fig. . The arrow shows the period at which APETx2 and RR were injected (3–5 d after RCS). Two-way ANOVA with repeated measures (see Supplementary Table ) followed by Bonferroni’s post hoc test. ## : p < 0.01 compared to the PBS group (main effect of the “drug”), $$, $$$: p < 0.01, 0.001 compared to “pre RCS”, *, **: p < 0.05, 0.01 compared to “pre Inj” in each drug group, &&&: p < 0.001 compared to PBS at the time point, APETx2 (APET) 0.73 μM and 2.2 μM reversed the mechanical withdrawal threshold decreased after RCS but RR neither 1.5 μM nor 15 μM did. ( B ) Vertical axis: ASIC3 mRNA and TRPV1 mRNA relative to GAPDH mRNA. DRGs were removed from rats 1 week after RCS. *: p < 0.05. Note that only ASIC3 mRNA level increased significantly after RCS. ( C ) Western blot analysis of ASIC3 protein expression in DRGs of RCS and control (CTR) rats. Blots from two rats for each group were represented. GAPDH was used as an internal control. ( D ) ASIC3 protein levels in DRGs quantified from the Western blot (n = 6 for each group). Data were shown as relative values to GAPDH. * : p < 0.05. (See Supplementary Table for statistics summary for ( B ) and ( D )).

Article Snippet: The upper piece of membrane was probed with anti-ASIC3 antibody raised in rabbit (1:200, GeneTex, Irvine, CA, USA) and goat anti-rabbit IgG conjugated with HRP (1:2000, Sigma Aldrich), and the lower piece with mouse anti-GAPDH antibody (1:10,000, GeneTex) and goat anti-mouse IgG conjugated with HRP (1:1000, Thermo Fisher Scientific Inc.) to use as loading control.

Techniques: Expressing, Injection, Western Blot